结核分枝杆菌早期分泌抗原靶6家族其他蛋白抗原的研究进展

doi:10.3969/j.issn.1000-6621.2014.06.019

摘要/Abstract

摘要： 目前结核分枝杆菌的致病机制和宿主的防御机制尚不十分清楚，结核分枝杆菌蛋白抗原生物学特性的研究有助于该问题的阐明，将为结核病疫苗、免疫诊断试剂和新药的开发奠定基础。早期分泌抗原靶6（ESAT-6）家族蛋白是一类小分子蛋白，呈螺旋状结构，它们通过ESAT-6分泌系统（ESAT-6 secretion system，ESX）分泌到细胞外。该家族共有23个成员（EsxA~W），其在基因组上相邻排列的2个蛋白编码基因形成11个类操纵子结构的基因对。该家族蛋白参与宿主与致病菌之间的相互作用，是人免疫系统识别的优势抗原，大多数是T细胞优势抗原，在结核分枝杆菌致病机制和机体的免疫保护机制方面起关键作用。鉴于EsxA和EsxB的研究概况国内外报道较多，而其他ESAT-6家族成员研究报道较少。因此，笔者着重概要地介绍ESAT-6家族其他成员的国内外研究进展情况，为进一步的深入研究和应用奠定基础。

关键词: 抗原, 细菌, 细菌蛋白质类, DNA结合蛋白质类, 原癌基因蛋白质类, 转录因子, 分枝杆菌, 结核

Abstract: At present, the pathogenesis of Mycobacterium tuberculosis (Mtb) and the defense mechanism of the host are not very clear. The study on the biological characteristics of Mtb protein antigen will contribute to understand these problems, and lay the foundation for the development of new tuberculosis vaccine, immune diagnostic reagents and drugs. The early secreted antigenic target 6 (ESAT-6) family proteins is a class of small molecular proteins with spiral structure, which exports the cell through the ESX secretion system. This family includes 23 members (EsxA-W), in which the genes coding two adjacent proteins in the genome form 11 gene pairs liking operon structure. These proteins involve in the interaction between the host and pathogen, are the immunodominant antigens in the recognition of human immune system, in which most of them are immunodominant T cell antigens, play a key role on the pathogenesis of Mtb and immune protection mechanism of the individual. There have been more reports on the EsxA and EsxB, but the researches on other members of the ESAT-6 family were less reported. Therefore, in this paper we briefly introduced the research progress of other ESAT-6 family members in order to lay the foundation for further research.

Key words: Antigens, bacterial, Bacterial proteins, DNA-binding proteins, Proto-oncogene proteins, Transcription factors, Mycobacterium tuberculosis

阳幼荣，吴雪琼. 结核分枝杆菌早期分泌抗原靶6家族其他蛋白抗原的研究进展[J]. 中国防痨杂志, 2014, 36(6): 503-508. doi: 10.3969/j.issn.1000-6621.2014.06.019

YANG You-rong, WU Xue-qiong. Research progress on ESAT-6 family protein of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2014, 36(6): 503-508. doi: 10.3969/j.issn.1000-6621.2014.06.019

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